Welcome
Our main area of investigation is the discovery of the molecular players that through epigenetic alterations of the genome are involved in the regulation of pluripotency and stemness in cancer stem cells and primordial germ cells.
© Dr. Hesam Dehghani 2021
Featured Article
DEVELOPMENT OF A GENOME KNOCK-IN APPROACH
A CRISPR/dCAS9 system using dual sgRNAs
© Dr. Hesam Dehghani 2021
Welcome
Our main area of investigation is the discovery of the molecular players that through epigenetic alterations of the genome are involved in the regulation of pluripotency and stemness in cancer stem cells and primordial germ cells.
© Dr. Hesam Dehghani 2021
Featured Article
DEVELOPMENT OF A GENOME KNOCK-IN APPROACH
A CRISPR/dCAS9 system using dual sgRNAs
Welcome
The Dehghani lab investigates the molecular players that through epigenetic alterations of the genome are involved in the regulation of pluripotency and stemness in cancer stem cells and primordial germ cells.
© Dr. Hesam Dehghani 2021
Featured Article
DEVELOPMENT OF A GENOME KNOCK-IN APPROACH
A CRISPR/dCAS9 system using dual sgRNAs
Our Research
The epigenetics of pluripotency and
stemness
Our lab is aimed to identify large noncoding RNAs and retrotransposons that by mediating epigenetic modifications could function in pluripotency, stemness, and cancer states.Development of molecules for efficient
genome transgenesis
Our goal is to design and build molecular tools that can be used for efficient knockout and knockin transgenesis of the genome, and degradation of lncRNAs.Production of biopharmaceuticals in
genome-engineered chicken/vaccine
development
Our lab for the first time in Iran has been able to produce genome- engineered chicken by genome editing of primordial germ cells. The two germline chimeric roosters has been named “Ghand” and “Nabat”. We have used a collection of cellular and molecular techniques to perform genetic modification of the in vitro cultured primordial germ cells and to return them back to the embryo to support development.
I
© Dr. Hesam Dehghani 2021
Our Research
The epigenetics of pluripotency
and stemness
Our lab is aimed to identify large noncoding RNAs and retrotransposons that by mediating epigenetic modifications could function in pluripotency, stemness, and cancer states.Development of molecules for
efficient genome transgenesis
outcomes
Our goal is to design and build molecular tools that can be used for efficient knockout and knockin transgenesis of the genome, and degradation of lncRNAs.
Production of biopharmaceuticals
in genome-engineered
chicken/vaccine development
Our lab for the first time in Iran has been able to produce genome-engineered chicken by genome editing of primordial germ cells. The two germline chimeric roosters has been named “Ghand” and “Nabat”. We have used a collection of cellular and molecular techniques to perform genetic modification of the in vitro cultured primordial germ cells and to return them back to the embryo to support development.
© Dr. Hesam Dehghani 2021
Our Research
The epigenetics of
pluripotency and
stemness
Our lab is aimed to identify large noncoding RNAs and retrotransposons that by mediating epigenetic modifications could function in pluripotency, stemness, and cancer states.Development of
molecules for
efficient genome
transgenesis
Our goal is to design and build molecular tools that can be used for efficient knockout and knockin transgenesis of the genome, and degradation of lncRNAs.
Production of biopharmaceuticals in
genome-engineered chicken/vaccine
development
Our lab for the first time in Iran has been able to produce genome-engineered chicken by genome editing of primordial germ cells. The two germline chimeric roosters has been named “Ghand” and “Nabat”. We have used a collection of cellular and molecular techniques to perform genetic modification of the in vitro cultured primordial germ cells and to return them back to the embryo to support development.
© Dr. Hesam Dehghani 2021
Lab members
Dr. Hesam Dehghani
Principal investigator
Professor | Molecular Developmental Biology Stem Cells & Regenerative Medicine Group, Research Institute of Biotechnology, Department of Basic Sciences, Faculty of Veterinary Medicine Division of Biotechnology, Faculty of Veterinary Medicine Ferdowsi University of Mashhad Email: dehghani@um.ac.ir ORCID public view Web of Science ResearcherID (Publons) researcher page Google Scholar researcher page Scopus author details
Current lab members
PhD candidates Nima Dehdilani, DVM, PhD candidate Kadijeh Hashemi, BSc, MSc, PhD candidate Zahra Hosseininia, BSc, MSc, PhD candidate Ali Miraghel, BSc, MSc, PhD candidate Mahereh Rezazadeh, BSc, MSc, PhD candidate Milad Shademan, BSc, MSc, PhD candidate Somayeh Sheikh, BSc, MSc, PhD candidate Emad Tayebbi, BSc, MSc, PhD candidate Sara Yousefi, BSc, MSc, PhD candidate Morteza Zahedi, BSc, MSc, PhD candidate Postdoctoral fellows Khadijeh Zare, BSc, MSc, PhD
MSc graduates
Mohammad Ali Foroughi, BSc, MSc (2010)
Sara Yousefi, BSc, MSc (2012)
Zahra Hoseininia, BSc, MSc (2013)
Mohammad Mehmandoost, MSc (2015)
Azam Naseri, BSc, MSc (2019)
Taraneh Ebnalnassir, BSc, MSc (2020)
Research assistants
Nima Saeedi BSc (2020)
Farnaz Fazaeli BSc (2020)
DVM & DVSc graduates
Mahboubeh Ebrahimian, DVM (2009)
Mahsa Mojtahedzadeh, DVM (2010)
Vajiheh Soltaninezhad, DVM (2010)
Zahra Baghestani, DVM (2012)
Saeed Saberol-shoara, DVM (2012)
Amin Nozari, DVM (2012)
Hedieh Rahimian, DVM (2017)
Munes Yadegari, DVM (2018)
Elaheh Khazaee, DVM, DVSc (2018)
© Dr. Hesam Dehghani 2021
© Dr. Hesam Dehghani 2021
Former lab members
PhD graduates Mohsen Naeimipour, DVM, PhD (2013) Sara Soltanian, BSc, MSc, PhD (2014) Elias Tabatabaeizadeh, DVM, PhD (2014) Mohammad Mehmandoost, MSc (2015) Masoumeh Es-haghi, BSc, MSc, PhD (2016) Khadijeh Zare, BSc, MSc, PhD (2019) Amin Tavassoli, BSc, MSc, PhD (2020) Safoura Soleimani, BSc, MSc, PhD (2020) Lena Goshayeshi, BSc, MSc, PhD (2021)
Lab members
Dr. Hesam Dehghani
Principal investigator
Professor | Molecular Developmental Biology Stem Cells & Regenerative Medicine Group, Research Institute of Biotechnology, Department of Basic Sciences, Faculty of Veterinary Medicine Division of Biotechnology, Faculty of Veterinary Medicine Ferdowsi University of Mashhad Email: dehghani@um.ac.ir ORCID public view Web of Science ResearcherID (Publons) researcher page Google Scholar researcher page Scopus author details 
© Dr. Hesam Dehghani 2021
Current lab members
PhD candidates Nima Dehdilani, DVM, PhD candidate Kadijeh Hashemi, BSc, MSc, PhD candidate Zahra Hosseininia, BSc, MSc, PhD candidate Ali Miraghel, BSc, MSc, PhD candidate Mahereh Rezazadeh, BSc, MSc, PhD candidate Milad Shademan, BSc, MSc, PhD candidate Somayeh Sheikh, BSc, MSc, PhD candidate Emad Tayebbi, BSc, MSc, PhD candidate Sara Yousefi, BSc, MSc, PhD candidate Morteza Zahedi, BSc, MSc, PhD candidate Postdoctoral fellows Khadijeh Zare, BSc, MSc, PhD
MSc graduates
Mohammad Ali Foroughi, BSc, MSc (2010)
Sara Yousefi, BSc, MSc (2012)
Zahra Hoseininia, BSc, MSc (2013)
Mohammad Mehmandoost, MSc (2015)
Azam Naseri, BSc, MSc (2019)
Taraneh Ebnalnassir, BSc, MSc (2020)
Research assistants
Nima Saeedi BSc (2020)
Farnaz Fazaeli BSc (2020)
DVM & DVSc graduates
Mahboubeh Ebrahimian, DVM (2009)
Mahsa Mojtahedzadeh, DVM (2010)
Vajiheh Soltaninezhad, DVM (2010)
Zahra Baghestani, DVM (2012)
Saeed Saberol-shoara, DVM (2012)
Amin Nozari, DVM (2012)
Hedieh Rahimian, DVM (2017)
Munes Yadegari, DVM (2018)
Elaheh Khazaee, DVM, DVSc (2018)
Former lab members
PhD graduates Mohsen Naeimipour, DVM, PhD (2013) Sara Soltanian, BSc, MSc, PhD (2014) Elias Tabatabaeizadeh, DVM, PhD (2014) Mohammad Mehmandoost, MSc (2015) Masoumeh Es-haghi, BSc, MSc, PhD (2016) Khadijeh Zare, BSc, MSc, PhD (2019) Amin Tavassoli, BSc, MSc, PhD (2020) Safoura Soleimani, BSc, MSc, PhD (2020) Lena Goshayeshi, BSc, MSc, PhD (2021)
Lab members
Dr. Hesam Dehghani
Principal investigator
Professor | Molecular Developmental Biology Stem Cells & Regenerative Medicine Group, Research Institute of Biotechnology, Department of Basic Sciences, Faculty of Veterinary Medicine Division of Biotechnology, Faculty of Veterinary Medicine Ferdowsi University of Mashhad Email: dehghani@um.ac.ir ORCID public view Web of Science ResearcherID (Publons) researcher page Google Scholar researcher page Scopus author details 
Former lab members
PhD graduates Mohsen Naeimipour, DVM, PhD (2013) Sara Soltanian, BSc, MSc, PhD (2014) Elias Tabatabaeizadeh, DVM, PhD (2014) Mohammad Mehmandoost, MSc (2015) Masoumeh Es-haghi, BSc, MSc, PhD (2016) Khadijeh Zare, BSc, MSc, PhD (2019) Amin Tavassoli, BSc, MSc, PhD (2020) Safoura Soleimani, BSc, MSc, PhD (2020) Lena Goshayeshi, BSc, MSc, PhD (2021)
MSc graduates
Mohammad Ali Foroughi, BSc, MSc (2010)
Sara Yousefi, BSc, MSc (2012)
Zahra Hoseininia, BSc, MSc (2013)
Mohammad Mehmandoost, MSc (2015)
Azam Naseri, BSc, MSc (2019)
Taraneh Ebnalnassir, BSc, MSc (2020)
Research assistants
Nima Saeedi BSc (2020)
Farnaz Fazaeli BSc (2020)
DVM & DVSc graduates
Mahboubeh Ebrahimian, DVM (2009)
Mahsa Mojtahedzadeh, DVM (2010)
Vajiheh Soltaninezhad, DVM (2010)
Zahra Baghestani, DVM (2012)
Saeed Saberol-shoara, DVM (2012)
Amin Nozari, DVM (2012)
Hedieh Rahimian, DVM (2017)
Munes Yadegari, DVM (2018)
Elaheh Khazaee, DVM, DVSc (2018)
Current lab members
PhD candidates Nima Dehdilani, DVM, PhD candidate Kadijeh Hashemi, BSc, MSc, PhD candidate Zahra Hosseininia, BSc, MSc, PhD candidate Ali Miraghel, BSc, MSc, PhD candidate Mahereh Rezazadeh, BSc, MSc, PhD candidate Milad Shademan, BSc, MSc, PhD candidate Somayeh Sheikh, BSc, MSc, PhD candidate Emad Tayebbi, BSc, MSc, PhD candidate Sara Yousefi, BSc, MSc, PhD candidate Morteza Zahedi, BSc, MSc, PhD candidate Postdoctoral fellows Khadijeh Zare, BSc, MSc, PhD
© Dr. Hesam Dehghani 2021
See our publications in PubMed, ResearcherID, Google Scholar
Selected Publications
Development of molecules for
efficient genome transgenesis
•
Goshayeshi L, Yousefi Taemeh S, Dehdilani N, Nasiri M, Ghahramani Seno MM, Dehghani H. CRISPR/dCas9-mediated
transposition with specificity and efficiency of site-directed genomic insertions. FASEB J. 2021 Feb;35(2):e21359. doi:
10.1096/fj.202001830RR.
•
Zare K, Shademan M, Ghahramani Seno MM, Dehghani H. CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 lncRNA
Gene in Cancer Cells. Biol Proced Online. 2018;20:21. doi: 10.1186/s12575-018-0086-5. eCollection 2018.
•
Soleymani S, Tavassoli A, Hashemi Tabar G, Kalidari GA, Dehghani H. Design, development, and evaluation of the efficacy
of a nucleic acid-free version of a bacterial ghost candidate vaccine against avian pathogenic E. coli (APEC) O78:K80
serotype. Vet Res. 2020 Dec 9;51(1):144. doi: 10.1186/s13567-020-00867-w.
•
Tavassoli A, Soleymani S, Haghparast A, Hashemi Tabar G, Bassami MR, Dehghani H. Reverse Genetics Assembly of
Newcastle Disease Virus Genome Template Using Asis-Sal-Pac BioBrick Strategy. Biol Proced Online. 2020;22:9. doi:
10.1186/s12575-020-00119-3. eCollection 2020.
•
Tabatabaeizadeh SE, Bassami MR, Haghparast A, Dehghani H. Employing XIAP to enhance the duration of antigen
expression and immunity against an avian influenza H5 DNA vaccine. Immunol Invest. 2015;44(3):199-215. doi:
10.3109/08820139.2014.988718.
Vaccine development
•
Shademan M, Zare K, Zahedi M, Mosannen Mozaffari H, Bagheri Hosseini H, Ghaffarzadegan K, Goshayeshi L, Dehghani
H. Promoter methylation, transcription, and retrotransposition of LINE-1 in colorectal adenomas and adenocarcinomas.
Cancer Cell Int. 2020;20:426. doi: 10.1186/s12935-020-01511-5. eCollection 2020.
•
Soltanian S, Dehghani H. BORIS: a key regulator of cancer stemness. Cancer Cell Int. 2018;18:154. doi: 10.1186/s12935-
018-0650-8. eCollection 2018. Review.
•
Es-Haghi M, Soltanian S, Dehghani H. Perspective: Cooperation of Nanog, NF-κΒ, and CXCR4 in a regulatory network for
directed migration of cancer stem cells. Tumour Biol. 2016 Feb;37(2):1559-65. doi: 10.1007/s13277-015-4690-6.
•
Fussner E, Ahmed K, Dehghani H, Strauss M, Bazett-Jones DP. Changes in chromatin fiber density as a marker for
pluripotency. Cold Spring Harb Symp Quant Biol. 2010;75:245-9. doi: 10.1101/sqb.2010.75.012.
•
Ahmed K, Dehghani H, Rugg-Gunn P, Fussner E, Rossant J, Bazett-Jones DP. Global chromatin architecture reflects
pluripotency and lineage commitment in the early mouse embryo. PLoS One. 2010 May 7;5(5):e10531. doi:
10.1371/journal.pone.0010531.
•
Efroni S, Duttagupta R, Cheng J, Dehghani H, Hoeppner DJ, Dash C, Bazett-Jones DP, Le Grice S, McKay RD, Buetow KH,
Gingeras TR, Misteli T, Meshorer E. Global transcription in pluripotent embryonic stem cells. Cell Stem Cell. 2008 May
8;2(5):437-47. doi: 10.1016/j.stem.2008.03.021.
•
Dehghani H, Dellaire G, Bazett-Jones DP. Organization of chromatin in the interphase mammalian cell. Micron.
2005;36(2):95-108. doi: 10.1016/j.micron.2004.10.003.
Chromatin and epigenetic basis of
stemness
See our publications in PubMed, ResearcherID, Google Scholar
Publications
Selected Publications
Development of molecules for efficient genome transgenesis
•
Goshayeshi L, Yousefi Taemeh S, Dehdilani N, Nasiri M, Ghahramani Seno MM, Dehghani H. CRISPR/dCas9-
mediated transposition with specificity and efficiency of site-directed genomic insertions. FASEB J. 2021
Feb;35(2):e21359. doi: 10.1096/fj.202001830RR.
•
Zare K, Shademan M, Ghahramani Seno MM, Dehghani H. CRISPR/Cas9 Knockout Strategies to Ablate CCAT1
lncRNA Gene in Cancer Cells. Biol Proced Online. 2018;20:21. doi: 10.1186/s12575-018-0086-5. eCollection
2018.
•
Shademan M, Zare K, Zahedi M, Mosannen Mozaffari H, Bagheri Hosseini H, Ghaffarzadegan K, Goshayeshi
L, Dehghani H. Promoter methylation, transcription, and retrotransposition of LINE-1 in colorectal
adenomas and adenocarcinomas. Cancer Cell Int. 2020;20:426. doi: 10.1186/s12935-020-01511-5.
eCollection 2020.
•
Soltanian S, Dehghani H. BORIS: a key regulator of cancer stemness. Cancer Cell Int. 2018;18:154. doi:
10.1186/s12935-018-0650-8. eCollection 2018. Review.
•
Es-Haghi M, Soltanian S, Dehghani H. Perspective: Cooperation of Nanog, NF-κΒ, and CXCR4 in a regulatory
network for directed migration of cancer stem cells. Tumour Biol. 2016 Feb;37(2):1559-65. doi:
10.1007/s13277-015-4690-6.
•
Fussner E, Ahmed K, Dehghani H, Strauss M, Bazett-Jones DP. Changes in chromatin fiber density as a
marker for pluripotency. Cold Spring Harb Symp Quant Biol. 2010;75:245-9. doi: 10.1101/sqb.2010.75.012.
•
Ahmed K, Dehghani H, Rugg-Gunn P, Fussner E, Rossant J, Bazett-Jones DP. Global chromatin architecture
reflects pluripotency and lineage commitment in the early mouse embryo. PLoS One. 2010 May
7;5(5):e10531. doi: 10.1371/journal.pone.0010531.
•
Efroni S, Duttagupta R, Cheng J, Dehghani H, Hoeppner DJ, Dash C, Bazett-Jones DP, Le Grice S, McKay RD,
Buetow KH, Gingeras TR, Misteli T, Meshorer E. Global transcription in pluripotent embryonic stem cells.
Cell Stem Cell. 2008 May 8;2(5):437-47. doi: 10.1016/j.stem.2008.03.021.
•
Dehghani H, Dellaire G, Bazett-Jones DP. Organization of chromatin in the interphase mammalian cell.
Micron. 2005;36(2):95-108. doi: 10.1016/j.micron.2004.10.003.
Chromatin and epigenetic basis of stemness
Vaccine development
•
Soleymani S, Tavassoli A, Hashemi Tabar G, Kalidari GA, Dehghani H. Design, development, and evaluation of
the efficacy of a nucleic acid-free version of a bacterial ghost candidate vaccine against avian pathogenic E.
coli (APEC) O78:K80 serotype. Vet Res. 2020 Dec 9;51(1):144. doi: 10.1186/s13567-020-00867-w.
•
Tavassoli A, Soleymani S, Haghparast A, Hashemi Tabar G, Bassami MR, Dehghani H. Reverse Genetics
Assembly of Newcastle Disease Virus Genome Template Using Asis-Sal-Pac BioBrick Strategy. Biol Proced
Online. 2020;22:9. doi: 10.1186/s12575-020-00119-3. eCollection 2020.
•
Tabatabaeizadeh SE, Bassami MR, Haghparast A, Dehghani H. Employing XIAP to enhance the duration of
antigen expression and immunity against an avian influenza H5 DNA vaccine. Immunol Invest.
2015;44(3):199-215. doi: 10.3109/08820139.2014.988718.
© Dr. Hesam Dehghani 2021
Publications
See our publications in PubMed, ResearcherID, Google Scholar
Publications
© Dr Hesam Dehghani 2020
•
Goshayeshi L, Yousefi Taemeh S, Dehdilani N, Nasiri M, Ghahramani Seno MM,
Dehghani H. CRISPR/dCas9-mediated transposition with specificity and efficiency
of site-directed genomic insertions. FASEB J. 2021 Feb;35(2):e21359. doi:
10.1096/fj.202001830RR.
•
Zare K, Shademan M, Ghahramani Seno MM, Dehghani H. CRISPR/Cas9
Knockout Strategies to Ablate CCAT1 lncRNA Gene in Cancer Cells. Biol Proced
Online. 2018;20:21. doi: 10.1186/s12575-018-0086-5. eCollection 2018.
Development of molecules for efficient genome transgenesis
Chromatin and epigenetic basis of stemness
•
Shademan M, Zare K, Zahedi M, Mosannen Mozaffari H, Bagheri Hosseini H,
Ghaffarzadegan K, Goshayeshi L, Dehghani H. Promoter methylation,
transcription, and retrotransposition of LINE-1 in colorectal adenomas and
adenocarcinomas. Cancer Cell Int. 2020;20:426. doi: 10.1186/s12935-020-01511-5.
eCollection 2020.
•
Soltanian S, Dehghani H. BORIS: a key regulator of cancer stemness. Cancer Cell
Int. 2018;18:154. doi: 10.1186/s12935-018-0650-8. eCollection 2018. Review.
•
Es-Haghi M, Soltanian S, Dehghani H. Perspective: Cooperation of Nanog, NF-κΒ,
and CXCR4 in a regulatory network for directed migration of cancer stem cells.
Tumour Biol. 2016 Feb;37(2):1559-65. doi: 10.1007/s13277-015-4690-6.
•
Fussner E, Ahmed K, Dehghani H, Strauss M, Bazett-Jones DP. Changes in
chromatin fiber density as a marker for pluripotency. Cold Spring Harb Symp
Quant Biol. 2010;75:245-9. doi: 10.1101/sqb.2010.75.012.
•
Ahmed K, Dehghani H, Rugg-Gunn P, Fussner E, Rossant J, Bazett-Jones DP. Global
chromatin architecture reflects pluripotency and lineage commitment in the early
mouse embryo. PLoS One. 2010 May 7;5(5):e10531. doi:
10.1371/journal.pone.0010531.
•
Efroni S, Duttagupta R, Cheng J, Dehghani H, Hoeppner DJ, Dash C, Bazett-Jones
DP, Le Grice S, McKay RD, Buetow KH, Gingeras TR, Misteli T, Meshorer E. Global
transcription in pluripotent embryonic stem cells. Cell Stem Cell. 2008 May
8;2(5):437-47. doi: 10.1016/j.stem.2008.03.021.
•
Dehghani H, Dellaire G, Bazett-Jones DP. Organization of chromatin in the
interphase mammalian cell. Micron. 2005;36(2):95-108. doi:
10.1016/j.micron.2004.10.003.
Vaccine development
•
Soleymani S, Tavassoli A, Hashemi Tabar G, Kalidari GA, Dehghani H. Design,
development, and evaluation of the efficacy of a nucleic acid-free version of a
bacterial ghost candidate vaccine against avian pathogenic E. coli (APEC)
O78:K80 serotype. Vet Res. 2020 Dec 9;51(1):144. doi: 10.1186/s13567-020-
00867-w.
•
Tavassoli A, Soleymani S, Haghparast A, Hashemi Tabar G, Bassami MR,
Dehghani H. Reverse Genetics Assembly of Newcastle Disease Virus Genome
Template Using Asis-Sal-Pac BioBrick Strategy. Biol Proced Online. 2020;22:9.
doi: 10.1186/s12575-020-00119-3. eCollection 2020.
•
Tabatabaeizadeh SE, Bassami MR, Haghparast A, Dehghani H. Employing XIAP
to enhance the duration of antigen expression and immunity against an avian
influenza H5 DNA vaccine. Immunol Invest. 2015;44(3):199-215. doi:
10.3109/08820139.2014.988718.
© Dr. Hesam Dehghani 2021
Contact Us
Research Institute of Biotechnology Shohada Building Ferdowsi University of Mashhad Azadi Square, Mashhad, Iran 91779-48974 Dr. Hesam Dehghani Email: dehghani@um.ac.ir |hesamdehghani@gmail.com Tel: +98 (51) 3880-3795The coordinates of our location
Contact Us
Research Institute of Biotechnology Shohada Building Ferdowsi University of Mashhad Azadi Square, Mashhad, Iran 91779-48974 Dr. Hesam Dehghani Email: dehghani@um.ac.ir |hesamdehghani@gmail.com Tel: +98 (51) 3880-3795The coordinates of our location
© Dr. Hesam Dehghani 2021
Contact Us
Research Institute of Biotechnology Shohada Building Ferdowsi University of Mashhad Azadi Square, Mashhad, Iran 91779-48974 Dr. Hesam Dehghani Email: dehghani@um.ac.ir |hesamdehghani@gmail.com Tel: +98 (51) 3880-3795The coordinates of our location
© Dr Hesam Dehghani 2021
