Welcome

The Dehghani lab investigates the molecular players that through epigenetic alterations of the genome are involved in the regulation of pluripotency and stemness in cancer stem cells and primordial germ cells.

BORIS as an epigenetic modifier BORIS: a key regulator of cancer stemness 

CRISPR/Cas9 knockout strategies CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 lncRNA Gene in Cancer Cells 

Differentially expressed genes in CCAT1-ko cells Expression profile analysis of two antisense lncRNAs to improve prognosis prediction of colorectal adenocarcinoma 

Featured publications

Stra8-independent initiation of meiosis, only in the presence of mesonephros Meiotic initiation in chicken germ cells is regulated by Cyp26b1 and mesonephros 

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Welcome

BORIS as an epigenetic modifier BORIS: a key regulator of cancer stemness 

Differentially expressed genes in CCAT1-ko cells Expression profile analysis of two antisense lncRNAs to improve prognosis prediction of colorectal adenocarcinoma 

Featured publications

Stra8-independent initiation of meiosis, only in the presence of mesonephros Meiotic initiation in chicken germ cells is regulated by Cyp26b1 and mesonephros 

CRISPR/Cas9 knockout strategies CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 lncRNA Gene in Cancer Cells 

Made with Xara

Welcome

BORIS as an epigenetic modifier BORIS: a key regulator of cancer stemness 

Differentially expressed genes in CCAT1-ko cells Expression profile analysis of two antisense lncRNAs to improve prognosis prediction of colorectal adenocarcinoma 

Featured publications

Stra8-independent initiation of meiosis, only in the presence of mesonephros Meiotic initiation in chicken germ cells is regulated by Cyp26b1 and mesonephros 

CRISPR/Cas9 knockout strategies CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 lncRNA Gene in Cancer Cells 

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Our Research

The epigenetic regulation of pluripotency

Understanding the epigenetic

modifications responsible for

pluripotency and stemness

Our lab is aimed to identify large noncoding RNAs and retrotransposons that by mediating epigenetic modifications could function in pluripotency, stemness, and cancer states.

Development of molecules for efficient

genome transgenesis outcomes

Our goal is to design and build molecular tools that can be used for efficient knockout and knockin transgenesis of the genome, and degradation of lncRNAs.

Production of biopharmaceuticals in

genome-engineered chicken

Our lab for the first time in Iran has been able to produce genome- engineered chicken by genome editing of primordial germ cells. The two germline chimeric roosters has been named “Ghand” and “Nabat”. We have used a collection of cellular and molecular techniques to perform genetic modification of the in vitro cultured primordial germ cells and to return them back to the embryo to support development.

I

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Our Research

The epigenetic regulation of pluripotency

Understanding the epigenetic

modifications responsible for

pluripotency and stemness

Our lab is aimed to identify large noncoding RNAs and retrotransposons that by mediating epigenetic modifications could function in pluripotency, stemness, and cancer states.

Development of molecules for

efficient genome transgenesis

outcomes

Our goal is to design and build molecular tools that can be used for efficient knockout and knockin transgenesis of the genome, and degradation of lncRNAs.

Production of biopharmaceuticals

in genome-engineered chicken

Our lab for the first time in Iran has been able to produce genome-engineered chicken by genome editing of primordial germ cells. The two germline chimeric roosters has been named “Ghand” and “Nabat”. We have used a collection of cellular and molecular techniques to perform genetic modification of the in vitro cultured primordial germ cells and to return them back to the embryo to support development.

Made with Xara

Our Research

The epigenetic regulation of pluripotency

Understanding the

epigenetic

modifications

responsible for

pluripotency and

stemness

Our lab is aimed to identify large noncoding RNAs and retrotransposons that by mediating epigenetic modifications could function in pluripotency, stemness, and cancer states.

Development of

molecules for

efficient genome

transgenesis

outcomes

Our goal is to design and build molecular tools that can be used for efficient knockout and knockin transgenesis of the genome, and degradation of lncRNAs.

Production of

biopharmaceuticals

in genome-

Lab members

Dr. Hesam Dehghani

Principal investigator

Professor | Molecular Developmental Biology Stem Cells & Regenerative Medicine Group, Research Institute of Biotechnology, Department of Basic Sciences, Faculty of Veterinary Medicine Division of Biotechnology, Faculty of Veterinary Medicine Ferdowsi University of Mashhad Email: dehghani@um.ac.ir ORCID public view  Web of Science ResearcherID (Publons) researcher page  Google Scholar researcher page  Scopus author details 

Current lab members

PhD candidates Nima Dehdilani, DVM, PhD candidate  Lena Goshayeshi, BSc, MSc, PhD candidate  Kadijeh Hashemi, BSc, MSc, PhD candidate  Zahra Hosseininia, BSc, MSc, PhD candidate  Ali Miraghel, BSc, MSc, PhD candidate  Mahereh Rezazadeh, BSc, MSc, PhD candidate  Milad Shademan, BSc, MSc, PhD candidate  Somayeh Sheikh, BSc, MSc, PhD candidate  Safoura Soleymani, BSc, MSc, PhD candidate  Emad Tayebbi, BSc, MSc, PhD candidate  Sara Yousefi, BSc, MSc, PhD candidate  Morteza Zahedi, BSc, MSc, PhD candidate  Postdoctoral fellows Khadijeh Zare, BSc, MSc, PhD  Research assistants Nima Saeedi BSc Farnaz Fazaeli BSc

Former lab members

PhD graduates Mohsen Naeimipour, DVM, PhD (2013) Sara Soltanian, BSc, MSc, PhD (2014) Elias Tabatabaeizadeh, DVM, PhD (2014) Mohammad Mehmandoost, MSc (2015) Masoumeh Es-haghi, BSc, MSc, PhD (2016) Khadijeh Zare, BSc, MSc, PhD (2019) Amin Tavassoli, BSc, MSc, PhD (2020)

MSc graduates Mohammad Ali Foroughi, BSc, MSc (2010) Sara Yousefi, BSc, MSc (2012) Zahra Hoseininia, BSc, MSc (2013) Mohammad Mehmandoost, MSc (2015) Azam Naseri, BSc, MSc (2019) Taraneh Ebnalnassir, BSc, MSc (2020)

DVM & DVSc graduates Mahboubeh Ebrahimian, DVM (2009) Mahsa Mojtahedzadeh, DVM (2010) Vajiheh Soltaninezhad, DVM (2010) Zahra Baghestani, DVM (2012) Saeed Saberol-shoara, DVM (2012) Amin Nozari, DVM (2012) Hedieh Rahimian, DVM (2017) Munes Yadegari, DVM (2018) Elaheh Khazaee, DVM, DVSc (2018)

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Lab members

Dr. Hesam Dehghani

Principal investigator

Current lab members

Former lab members

Made with Xara

Lab members

Dr. Hesam Dehghani

Principal investigator

Current lab members

Former lab members

Home
research
members
publications
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See our publications in PubMed, ResearcherID, Google Scholar

Publications

Selected Publications

(1) Tavassoli, A.; Soleymani, S.; Haghparast, A.; Hashemi Tabar, G.; Bassami, M. R.; Dehghani, H. Reverse Genetics Assembly of Newcastle Disease Virus Genome Template Using Asis-Sal-Pac BioBrick Strategy. Biol Proced Online 2020, 22, 9. https://doi.org/10.1186/s12575-020-00119-3. (2) Hosseininia, Z.; Soltanian, S.; Mahdavi-Shahri, N.; Dehghani, H. Data on Analysis of OCC-1 Transcript Levels in Pluripotent and Differentiated States of P19 Cells. Data Brief 2020, 29, 105367. https://doi.org/10.1016/j.dib.2020.105367. (3) Yousefi Taemeh, S.; Mahdavi Shahri, N.; Lari, R.; Bahrami, A. R.; Dehghani, H. Meiotic Initiation in Chicken Germ Cells Is Regulated by Cyp26b1 and Mesonephros. J Exp Zool B Mol Dev Evol 2019, 332 (7), 269–278. https://doi.org/10.1002/jez.b.22904. (4) Shademan, M.; Naseri Salanghuch, A.; Zare, K.; Zahedi, M.; Foroughi, M. A.; Akhavan Rezayat, K.; Mosannen Mozaffari, H.; Ghaffarzadegan, K.; Goshayeshi, L.; Dehghani, H. Expression Profile Analysis of Two Antisense LncRNAs to Improve Prognosis Prediction of Colorectal Adenocarcinoma. Cancer Cell Int 2019, 19, 278. https://doi.org/10.1186/s12935-019-1000-1. (5) Zare, K.; Shademan, M.; Ghahramani Seno, M. M.; Dehghani, H. CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 LncRNA Gene in Cancer Cells. Biol Proced Online 2018, 20, 21. https://doi.org/10.1186/s12575-018-0086-5. (6) Soltanian, S.; Dehghani, H. BORIS: A Key Regulator of Cancer Stemness. Cancer Cell Int 2018, 18, 154. https://doi.org/10.1186/s12935-018-0650-8. (7) Khazaee, E.; Farzaneh, N.; Mirshokraei, P.; Tabatabaeizadeh, S.-E.; Dehghani, H. Expression of Endogenous Retroviruses in Pre-Implantation Stages of Bovine Embryo. Reprod Domest Anim 2018, 53 (6), 1405–1414. https://doi.org/10.1111/rda.13269. (8) Es-Haghi, M.; Soltanian, S.; Dehghani, H. Perspective: Cooperation of Nanog, NF-ΚΒ, and CXCR4 in a Regulatory Network for Directed Migration of Cancer Stem Cells. Tumour Biol 2016, 37 (2), 1559–1565. https://doi.org/10.1007/s13277-015-4690-6. (9) Es-Haghi, M.; Bassami, M.; Dehghani, H. Construction and Quantitative Validation of Chicken CXCR4 Expression Reporter. Mol Biotechnol 2016, 58 (3), 202–211. https://doi.org/10.1007/s12033-016-9917-2. (10) Tabatabaeizadeh, S.-E.; Bassami, M. R.; Haghparast, A.; Dehghani, H. Employing XIAP to Enhance the Duration of Antigen Expression and Immunity against an Avian Influenza H5 DNA Vaccine. Immunol Invest 2015, 44 (3), 199–215. https://doi.org/10.3109/08820139.2014.988718. (11) Naeemipour, M.; Dehghani, H.; Bassami, M.; Bahrami, A. Expression Dynamics of Pluripotency Genes in Chicken Primordial Germ Cells before and after Colonization of the Genital Ridges. Mol Reprod Dev 2013, 80 (10), 849–861. https://doi.org/10.1002/mrd.22216. (12) Fussner, E.; Ahmed, K.; Dehghani, H.; Strauss, M.; Bazett-Jones, D. P. Changes in Chromatin Fiber Density as a Marker for Pluripotency. Cold Spring Harb Symp Quant Biol 2010, 75, 245–249. https://doi.org/10.1101/sqb.2010.75.012. (13) Ebrahimian, M.; Mojtahedzadeh, M.; Bazett-Jones, D.; Dehghani, H. Transcript Isoforms of Promyelocytic Leukemia in Mouse Male and Female Gametes. Cells Tissues Organs 2010, 192 (6), 374–381. https://doi.org/10.1159/000319466. (14) Ahmed, K.; Dehghani, H.; Rugg-Gunn, P.; Fussner, E.; Rossant, J.; Bazett-Jones, D. P. Global Chromatin Architecture Reflects Pluripotency and Lineage Commitment in the Early Mouse Embryo. PLoS One 2010, 5 (5), e10531. https://doi.org/10.1371/journal.pone.0010531. (15) Efroni, S.; Duttagupta, R.; Cheng, J.; Dehghani, H.; Hoeppner, D. J.; Dash, C.; Bazett-Jones, D. P.; Le Grice, S.; McKay, R. D. G.; Buetow, K. H.; Gingeras, T. R.; Misteli, T.; Meshorer, E. Global Transcription in Pluripotent Embryonic Stem Cells. Cell Stem Cell 2008, 2 (5), 437–447. https://doi.org/10.1016/j.stem.2008.03.021. (16) Bazett-Jones, D. P.; Li, R.; Fussner, E.; Nisman, R.; Dehghani, H. Elucidating Chromatin and Nuclear Domain Architecture with Electron Spectroscopic Imaging. Chromosome Res 2008, 16 (3), 397–412. https://doi.org/10.1007/s10577-008-1237-3. (17) Dellaire, G.; Eskiw, C. H.; Dehghani, H.; Ching, R. W.; Bazett-Jones, D. P. Mitotic Accumulations of PML Protein Contribute to the Re-Establishment of PML Nuclear Bodies in G1. J Cell Sci 2006, 119 (Pt 6), 1034–1042. https://doi.org/10.1242/jcs.02817. (18) Dellaire, G.; Ching, R. W.; Dehghani, H.; Ren, Y.; Bazett-Jones, D. P. The Number of PML Nuclear Bodies Increases in Early S Phase by a Fission Mechanism. J Cell Sci 2006, 119 (Pt 6), 1026–1033. https://doi.org/10.1242/jcs.02816. (19) Dehghani, H.; Reith, C.; Hahnel, A. C. Subcellular Localization of Protein Kinase C Delta and Epsilon Affects Transcriptional and Post-Transcriptional Processes in Four-Cell Mouse Embryos. Reproduction 2005, 130 (4), 453–465. https://doi.org/10.1530/rep.1.00572. (20) Dehghani, H.; Hahnel, A. C. Expression Profile of Protein Kinase C Isozymes in Preimplantation Mouse Development. Reproduction 2005, 130 (4), 441–451. https://doi.org/10.1530/rep.1.00571. (21) Dehghani, H.; Dellaire, G.; Bazett-Jones, D. P. Organization of Chromatin in the Interphase Mammalian Cell. Micron 2005, 36 (2), 95–108. https://doi.org/10.1016/j.micron.2004.10.003.