This lab is interested in three main research areas:

Production of recombinant proteins in transgenic chicken A collection of cellular and molecular techniques are exploited to culture and maintain primordial germ cells and to perform genome transgenesis in these cells. Specific molecular methods for purification of recombinant proteins from egg white are also being adopted. Transgenesis of primordial germ cells using different state of the art genome editing techniques are aimed at specific sites of the genome.
Understanding the epigenetic modifications responsible for pluripotency and stemness While the majority of investigations over the last few decades have focused on the role of protein-coding genes, recent studies show that in most cancers external factors such as environment, food, physical activity, age, and etc. are able to change the state of transcription using epigenetic mechanisms including genome methylation, post-translational modifications of histones, and large noncoding RNAs. Our lab is aimed to identify large noncoding RNAs and retrotransposons that may be functional and critical at specific stages of cancer.
Epigenetic editing & reprogramming Another active area of research in this lab is therapeutic epigenetic editing in cancer cells and epigenetic reprogramming of primordial germ cells.
  •     Culture, maintenance, and transgenesis of primordial germ cells
  •     Targeted genome transgenesis of chicken PGCs for production of recombinant proteins
  •     CRISPR Cas9-mediated genome knock-in, knock-out
  •     Cell line development
  •     Epigenetic characterization of cells during pluripotency, stemness, and cancer states
  •     Epigenetic editing