The Dehghani Lab
 

This lab is interested in two main research areas:
1) Production of recombinant proteins in transgenic chicken
A collection of cellular and molecular techniques are exploited to culture and maintain primordial germ cells and to perform genome transgenesis in these cells. Specific molecular methods for purification of recombinant proteins from egg white are also being adopted. The culture conditions and further use of primordial germ cells are all based on their pluripotency and migratory potential. Transgenesis of primordial germ cells using different state of the art genome editing techniques are aimed at specific sites of the genome.

2) Understanding the epigenetic modifications responsible for pluripotency and stemness & epigenetic editing of cancer cells
While the majority of investigations over the last few decades have focused on the role of protein-coding genes, recent studies show that in most cancers external factors such as environment, food, physical activity, age, and etc. are able to change the state of transcription using epigenetic mechanisms including genome methylation, post-translational modifications of histones, and large noncoding RNAs. Our lab is aimed to identify large noncoding RNAs and retrotransposons that may be functional and critical at specific stages of cancer. Another active area of research in this lab is therapeutic epigenetic editing in cancer cells.

Targeted genome transgenesis of chicken primordial germ cells for production of recombinant proteins
CRISPR Cas9-mediated genome knock-in
CRISPR Cas9-mediated genome knock-out
Culture, maintenance, and transgenesis of primordial germ cells
Cell line development
Epigenetic characterization of cells during pluripotency, stemness, and cancer states
Epigenetic editing

 
Production of recombinant proteins in transgenic chicken
Understanding the epigenetic modifications responsible for pluripotency and stemness
Epigenetic editing of cancer cells
 

Embryonic and Stem Cell Biology and Biotechnology Research Group
The Research Institute of Biotechnology
Ferdowsi University of Mashhad
Azadi Square, Mashhad, Iran
91779-48974
Email: dehghani@um.ac.ir; hesamdehghani@gmail.com


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2016 by The Dehghani lab

 

June 4, 2016